Figure 3.
Restoration of peroxisome biogenesis. (A) Latency of catalase activity in CHO-K1, ZP107, and 107P1 cells. Catalase: ○, CHO-K1; ▵, ZP107; □, 107P1. Lactate dehydrogenase in ZP107 is shown by ⋄. Relative free enzyme activity is expressed as a percentage of the total activity measured in the presence of 1% Triton X-100 (9). The results represent a mean of duplicate assays. (B) Biogenesis of peroxisomal proteins. Cell lysates (≈1.8 × 105 cells) were subjected to SDS/PAGE and transferred to polyvinylidene difluoride (PVDF) membrane. Cell types are indicated at the top. Immunoblot analysis used rabbit antibodies to rat acyl-CoA oxidase (AOx) and 3-ketoacyl-CoA thiolase (Thiolase). Arrows show the positions of AOx polypeptide components A, B, and C; open and solid arrowheads indicate a larger precursor (P) and mature protein (M) of 3-ketoacyl-CoA thiolase, respectively. Dots indicate nonspecific bands.