Figure 4.

Molecular analyses of transgenic Physcomitrella generated by the construct outlined in Fig. 2C. Analyzed were water (lane 1), untransformed wild type (lane 2), three different transgenics with macrochloroplasts (lanes 3–5; compare Fig. 3 D–G), and a transgenic with wild type-like plastids (lane 6; compare Fig. 3 A–C), respectively. (A–C) DNA gel analysis of representative PCR reactions. Each pair of primers was specific for sequences from nptII (A), 5′-integration site (B) and 3′-integration site (C), respectively. See Fig. 2D for primer specifications. Sizes of PCR fragments were 700 bp (A), 373 bp (B), and 826 bp (C). (D) Representative RNA blot analysis. About 10 μg of total RNA (19) was isolated from plants and probed with the 1,173 bp PpftsZ subclone indicated in Fig. 2A. (E) RNA blot analysis. The same filter was reprobed with the nptII cassette indicated in Fig. 2B. Transcript sizes in nucleotides.