FIG. 2.

HSV-2 undergoes de novo viral gene expression in infected murine BMDC. CD11c⁺ BMDC were infected with wt HSV-1 or -2 at an MOI of 4 or left untreated and then incubated for up to 18 h. DC were collected from separate wells of a 24-h plate for each time point, mounted on slides (n = 5 slides/group/time point), fixed, permeabilized, and then stained with antibody specific for either the HSV early protein, ICP8 (383), or the late protein, gD (910). Shown are immunofluorescence microscope images of HSV-2-infected DC stained for ICP8 or gD at 2, 4, 6, or 16 h p.i. (magnification, ×100). No signal was detected in mock-infected cells after staining them for ICP8 or gD (UI) nor for ICP8 in UV-HSV-2-infected DC to 12 h p.i. (not shown). The results shown are from one of three independent experiments with similar results.