RFLP patterns generated by restriction enzyme digests with BamHI and BglII. Viral gradient DNA was digested with BamHI or BglII, and DNA fragments were separated on a 0.6% agarose gel. All HVP2 isolates were clearly distinct from both SA8 and BV. The lanes for both digests are as follows: lane M, DNA size marker; lanes 1 to 5, HVP2nv isolates OU1-76, OU3-1, A189164, OU3-18, and X313; lanes 6 to 9, HVP2ap isolates OU2-5, A951, OU4-8, and OU4-15; lane 10, SA8; lane 11, BV.