FIG. 5.

HCMV-infected monocytes exhibit a decreased ability for endocytosis and phagocytosis. To test whether HCMV-infected monocytes exhibited altered endocytosis, we tested the ability of dendritic cells and HCMV-infected monocytes to endocytose FITC-labeled dextran particles for 1 h after 7 days in culture. The cells were thereafter examined by flow cytometry to obtain histogram plots of dendritic cells and HCMV-infected monocytes. (A) Representative example of a histogram plot of the fluorescence values of dextran-FITC of HCMV-infected monocytes (dark gray line) and dendritic cells (light gray line) obtained by flow cytometry. (B) Statistical analysis of the mean channel values obtained by flow cytometry of FITC-dextran in dendritic cells (DC) and HCMV-infected monocytes (HCMV-Mo). The results represent mean and standard error of the mean values obtained from six separate experiments performed in duplicate. (C) The ability of the cells to phagocytose FITC-labeled yeast particles was tested at 7 days postinfection for 30 min at 37°C. Phagocytosis was monitored and quantified in a fluorescence microscope, and the results are presented as the number of cells that phagocytosed at least one yeast particle.