TABLE 1.
Antibody | Cell origin | kon (M−1s−1) | koff (s−1) | KD (M) |
---|---|---|---|---|
h667 | 667 hybridoma | 5.6 × 104 | 5.1 × 10−4 | 0.93 × 10−8 |
r667 | Cos-7 | 4.2 × 104 | 14.2 × 10−4 | 3.4 × 10−8 |
Kinetic constants were determined using the Biacore technology, as described in Materials and Methods, with Cos-7 and 667 hybridoma cell culture supernatants.