FIG. 5.

(A) RNA replication of AV-FL and the L mutants. Vero cells were electroporated with the RNA transcripts, and then total RNAs were extracted from the cells at the indicated times after electroporation. The total-RNA samples were dotted and probed with digoxigenin-labeled negative-sense viral RNA (nt 4790 to 5253). As controls, 10 and 1 ng of AV-FL transcripts were dotted. (B) Protein synthesis in Vero cells electroporated with the AV-FL or Δ114-163 RNA. At 2 or 5 h after electroporation, cells were labeled with [³⁵S]methionine-cysteine for 1 h. The cells were lysed, and then the proteins were analyzed by SDS-PAGE. Radioactive signals were detected with a phosphorimager. As markers, in vitro transcription-translation products of pAV-FL were used. Mock, mock transfected.