FIG. 6.
(A) Sedimentation analysis of viral and subviral particles generated in Vero cells electroporated with AV-FL or Δ114-163 RNA. Vero cells transfected with the RNAs were labeled with [35S]methionine-cysteine. At 6 h after electroporation, the labeled viral and subviral particles were collected and centrifuged through a 10 to 30% sucrose gradient. The gradient was fractionated, and the radioactivity in each fraction was counted with a liquid scintillation counter. (B) Proteins constructing virions and empty capsids. Parts of fractions containing virions (lane V) of the AV-FL virus and empty capsids (lane E) of the AV-FL virus and the Δ114-163 virus were analyzed by SDS-PAGE, and 35S-labeled proteins were visualized with a phosphorimager. As markers, in vitro transcription-translation products of pAV-FL were used (lane M).