TABLE 1.
Primers used for construction of mutants
| Mutant | Primer | Sequence | Position (nt) |
|---|---|---|---|
| pΔ43-135 | 1147P | 5′ CAAGCAAAGACCCCGGAGGAGCGT | 1147-1170 (plus strand) |
| 870M | 5′ AGAGAGATGGGGGTTGGTGTT | 850-870 (minus strand) | |
| pΔ4-53 | 901P | 5′ TGCCCGTTCTGTCAGTACGACG | 901-922 (plus strand) |
| 753-M | 5′ TGCAGCCATGACTCCGACATAAAAGG | 728-753 (minus strand) | |
| pΔ74-163 | 1234P | 5′ ACCACTAACCTCCAACGGCA | 1234-1253 (plus strand) |
| 963M | 5′ CTCGCCACGGAGAGACTCTGGGGA | 940-963 (minus strand) | |
| pΔ114-163 | 1234P | 5′ ACCACTAACCTCCAACGGCA | 1234-1253 (plus strand) |
| 1083M | 5′ GTATGTTTCAGGATAGAACCAGGAT | 1060-1083 (minus strand) | |
| pLfs | 766P | 5′ CGATCTGTGCTCGCTGCTGT | 766-785 (plus strand) |
| 764M | 5′ GAAACCCGTGTTGCAGCCATG | 764-744 (minus strand) | |
| 1234P | 5′ ACCACTAACCTCCAACGGCA | 1234-1254 (plus strand) | |
| Ains-1233M | 5′ TAGGGAGTGTGAGAGGGGGGGTGGAa | 1210-1233 (minus strand) |
a
The inserted nucleotide is underlined.