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. 2007 Dec 31;7:236. doi: 10.1186/1471-2407-7-236

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Copyright © 2007 Bu and Wan; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fenretinide increases the transcriptional activity of RARβ. Huh-7 cells were treated with fenretinide for 24 hours followed by ChIP assay. (A) RARβ and (B) CYP26A1 promoter fragments (harboring RARE) bound by RARβ were immunoprecipitated with a specific antibody against RARβ and amplified by PCR. Representative PCR results from two independent experiments were shown. The histograms depict the relative fold of PCR band intensity (after normalized to the corresponding input) between fenretinide treatment and DMSO treatment.