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. 2003 Oct;77(20):10872–10880. doi: 10.1128/JVI.77.20.10872-10880.2003

FIG. 1.

FIG. 1.

Transduction of EBV-LCL by Ad5/F35 Jagged-1 with subsequent activation of Notch downstream signal in T cells. (A) Results of real-time PCR showing overexpression of Jagged-1 mRNA (×64) in transduced EBV-LCL at 48 h (MOI = 100 PFU/cell) compared with that in nontransduced EBV-LCL. Data are means ± SD from three experiments. (B) Results of Western blotting showing the Jagged-1 protein of approximately 180 kDa 48 h after transduction. Human bone marrow stromal cells were used as the positive control. Shown are the results of one experiment that is representative of three. (C) Results of real-time PCR showing HES-1 and Deltex mRNA expression levels in T cells stimulated by nontransduced autologous EBV-LCL (filled columns) compared with those for autologous EBV-LCL Jagged-1 (open columns). Overexpression after stimulation with EBV-LCL Jagged-1 was observed at 12 h but not at 24 or 48 h. Data are means ± SD from three experiments. (D) Results of Western blotting showing an overexpression of the HES-1 protein (28 kDa) in T cells 24 h after stimulation with EBV-LCL Jagged-1. Shown are the results of one experiment that is representative of three. T cells/LCL-J1, T cells stimulated by autologous EBV-LCL transduced with Ad5/F35 Jagged-1; T cells/NT LCL, T cells stimulated by nontransduced autologous EBV-LCL.