FIG. 4.

Lymphocytes induced by EBV-LCL overexpressing Jagged-1 produce IL-10. Data indicate cytokine concentrations in culture supernatants at days 3 and 8 in three different culture conditions: PBMC plus autologous LCL cells (filled columns), PBMC plus autologous LCL cells transduced by Ad5/F35 EGFP (open columns), and PBMC plus autologous LCL cells transduced by Ad5/F35 Jagged-1 (hatched columns). 2 × 10⁶ PBMC and 5 × 10⁴ LCL cells were used in each condition. Data shown are means ± SD from four experiments. In all three conditions, no significant amount of IL-4 was detectable at either day 3 or day 8 and no significant amount of IL-2 was detectable at day 8. The increase of IL-10 was significant at days 3 and 8 (P = 0.002). No significant difference was observed for the other cytokines.