Table 4.
Name | Sequence | Location (bp)* |
---|---|---|
5′UTR-F1 | 5′-AGT TCT GCG CAT GCT CGG CTC G-3′ | 5′UTR |
E1F2 | 5′-CGC ACT GCT CTG GCT GGC GG-3′ | exon1 (96-115) |
E1F3 | 5′-GTG GCC GGT GCC CTA CAA-3′ | exon1 (162-179) |
I1R222 | 5′-ACA GTC ACT TTG GAA CTG GCT CAA CG-3′ | intron1 |
I1F11 | 5′-TAT AGA CTG TGT GCT CAG TC-3′ | intron1 |
E2F1 | 5′-GAT CCT TAT TGT CAA GCT A-3′ | exon2 (205-223) |
E2F2 | 5′-GAC CTC CTG GGA CAC TTG-3′ | exon2 (328-345) |
E2R2 | 5′-AAG TGT CCC AGG AGG TC-3′ | exon2 (328-344) |
I2R4 | 5′-TTG GTG TTC CTC TTC AGT TCA-3′ | intron2 |
I2R2 | 5′-CAG GCC CTG GGC ATG AAG TGA C-3′ | intron2 |
I2F3 | 5′-ATG AGG GCA GGG AGT TAG AG-3′ | intron2 |
E3F1 | 5′-ATG CAT GAT GCC ATT GGA TT-3′ | exon3 (352-371) |
E3R2 | 5′-CTA GAA CTA ACG TCC CAT TTT CCT-3′ | exon3 (533-556) |
I3R285 | 5'-CAG AGA ACA AAC TAG TGG TCA CC-3′ | intron3 |
I3F3 | 5′-TCA GAG GTA TCT TGT GCC TT-3′ | intron3 |
E4F1 | 5′-GCA TGT TTA ACA AAA TGG CAA-3′ | exon4 (575-595) |
E4R3 | 5′-GAA CTC TCT GTG TAT AAC CAC-3′ | exon4 (949-969) |
3′UTR-R1 | 5′-ACT GAT GTG CTG GTA GAA AGA A-3′ | 3′UTR |
Primer position based on the ovine CLN5 sequence submitted to Genbank (accession number NM_001082595)