Table 2.
Comparison of the total number of cells patched and the number of cells which exhibited APs in control (C37, C44), glutamate treated (G37, G44), serotonin treated (S37, S44), acetylcholine chloride treated (A37, A44), glutamate+serotonin treated (GS37, GS44), and glutamate+serotonin→ acetylcholine chloride treated (GSA44). 37 and 44 indicates 7 and 14 days after culturing the cells in the presence of neurotransmitters respectively. Percentages are indicated in parentheses.
| Total number of cells patched | Number of cells which did not fire any action potential (NP) | Number of cells which fired single action potentials (SAP) | Number of cells which fired double action potentials (DAP) | Number of cells which fired multiple action potentials (MAP) | |
|---|---|---|---|---|---|
| C37 | 24 | 17 | 7 (29.1%) | - | - |
| C44 | 49 | 35 | 14 (28.5%) | - | - |
| G37 | 42 | 28 | 14 (33.3%) | - | - |
| G44 | 14 | 8 | 6 (42.2%) | - | - |
| S37 | 17 | 10 | 7 (41.1%) | - | - |
| S44 | 25 | 14 | 11 (44.0%) | - | - |
| A37 | 30 | 25 | 5 (16.6%) | - | - |
| A44 | 31 | 25 | 6 (19.3%) | - | - |
| GS37 | 20 | 10 | 10 (50.0%) | - | - |
| GS44 | 27 | 11 | 14 (56.0%) | 2 (12.5%)** | - |
| GSA44* | 107 | 42 | 57 (60.7%) | 5 (7.6%)** | 3 (4.6%)*** |
A double classification Chi-squared test was used to test for multiple categories of data. We compare the X2 value with a tabulated χ2 with one degree of freedom. Our calculated X2 exceeds the tabulated χ2 value (3.84) for p = 0.05. We conclude that multiple neurotransmitter applications show significantly more electrically active cells as compared to the control.
Percentage of neurons as compared to the total number of electrically active neurons which fired double APs.
Percentage of neurons as compared to the total number of electrically active neurons which fired multiple APs.