Table 3.

Comparison of the electrical properties of the neurons which exhibited APs in control (C37, C44), glutamate treated (G37, G44), serotonin treated (S37, S44), acetylcholine chloride treated (A37, A44), glutamate+serotonin treated (GS37, GS44), and glutamate+serotonin→ acetylcholine chloride treated (GSA44). Since the neurotransmitters were added on day 30 after the cells were plated, the numbers 37 and 44 indicate 7 and 14 days respectively after culturing the cells in the presence of neurotransmitters. The values are expressed as Mean ± SE.

	C37	C44	G37	G44	S37	S44	A37	A44	GS37	GS44	GSA44	p
Days in vitro	37	44	37	44	37	44	37	44	37	44	44
Number of neurons (n)	7	14	14	6	7	11	5	6	10	16	65
Resting potential (Mv)	51.7±3.1	48.2±2.9	48.9±3.2	50.4±4.4	50.3±1.5	48.9±1.3	52.4±2.1	52.5±1.7	52.8±2.8	46.2±3.3	44.2±1.3	NS
Input resistance (mΩ)	163.3±12.1	169.1±11.1	124.1±16.6	110.3±16.5	130.9±9.2	136.9±12.9	128.2±7.1	131.7±10.7	144.5±8.9	278.9±74.0	237.9±23.1	NS
Capacitance (pF)	18.3±1.9	17.0±1.9	15.8±1.1	14.8±1.2	15.71±1.5	15.4±1.5	15.4±1.6	18.5±1.9	17.2±2.8	18.9±1.4	17.7±0.9	NS
Peak inward current (pA)	657.1±39.9	681.4±134.6	674.8±127.9	737.2±69.4	402.1±43.7	359.1±33.6	450.0±44.7	525.0±70.4	713.9±95.2	1376.4±105.2	1090.4±79.9	= 0.01
Peak outward current (pA)	770.7±43.1	833.53±110.3	883.6±166.2	855.0±77.3	829.3±70.2	745.5±67.5	605.0±37.4	625.0±61.6	768.6±83.2	1219.8±117.7	930.1±41.9	NS
Action potential height (mV)	26.4±2.4	16.6±1.3	18.1±2.3	20.2±3.4	20.7±1.5	16.9±1.3	18.8±2.6	20.2±2.4	18.4±1.8	25.6±2.4	23.84±1.1	NS
Action potential width (ms)	3.9±0.2	4.4±0.2	5.3±0.7	3.7±0.2	4.2±0.3	4.6±0.3	4.7±0.4	4.7±0.4	5.4±0.5	5.3±0.3	5.80±0.2	NS

p is calculated using a one-way analysis of variance. NS indicates not statistically significant.