FIG. 11.

Binding (A) (determined by ELISA) and neutralizing (B) antibodies induced in rhesus macaques following intranasal priming and intramuscular boosting regimen using o-gp140SF162ΔV2 in combination with LTK63 or LTR72. Briefly, two rhesus macaques were immunized five times at 3-week intervals with o-gp140SF162ΔV2 (300 μg) and LTK63 or LTR72 (100 μg) in a total volume of 800 μl (400 μl per nostril). After a resting period of 4 months, both the animals were immunized twice with 100 μg of o-gp140SF162ΔV2 in MF59 intramuscularly at 4-week intervals. For determining the neutralizing activity, samples were assayed at threefold dilutions in triplicate in M7-luc cells. Neutralization antibody (NAb) titers are the serum dilutions at which luciferase activity was reduced to 80% relative to the virus control wells (cells and virus).