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. 2003 Oct;77(20):11244–11259. doi: 10.1128/JVI.77.20.11244-11259.2003

FIG. 8.

FIG. 8.

Increased exposure of CD4i epitope recognized by the MAb 4.8d in o-gp140SF162ΔV2 upon binding to CD4, as determined by Biacore. (A) For this experiment, a reactive surface was prepared by capturing the MAb 4.8d on CM5 Bia-chip, and o-gp140SF162ΔV2 with (□) and without (▴) incubation with CD4 was flowed over this surface. Data were analyzed by using Biaevaluation 3.2. (B) Recognition of gp120SF162 and o-gp140SF162ΔV2 by MAbs 2F5 and 2G12 in Farr-Western assay. Lanes: 2 and 3, reactivity of gp120SF162 with 2G12 and 2F5, respectively; 4 and 5, recognition of o-gp140SF162ΔV2 by 2G12 and 2F5, respectively.