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Analysis of integration activity by Vpr-IN trans-complementation; 4 μg of pNL4-3D116A (black bars) or pNL4-3F185A (open bars) proviral DNA was cotransfected into 293T cells with 6 μg of pLR2P-vpr, pLR2P-vprIN1, or pLR2P-vprIN2 (controls) or with 6 μg of pLR2P-vprIN expression plasmids containing mutations in the IN2 coding region, as depicted. Infectivity was determined as described for Fig. 5.
