Figure 2.

Cytochrome f synthesis under control of the atpA 5′ UTR escapes the CES process. (A) Maps of the petA gene in wild-type and AFFF strains (Bg, BglII; N, NcoI). The heavily hatched box in the 5′ region of atpA denotes the sequence encoding the 25 first amino acids of the α subunit of the ATP synthase complex. (B) Newly synthesized cytochrome f and SUIV detected by pulse-labeling experiments in ΔpetA, wild-type, parental strains, and progeny of a representative tetrad from the cross AFFF × mcd1-F16. (C) Accumulation of cytochromes f and b₆, detected with specific antibodies in the same strains.