Figure 3.

petA 5′ UTR confers CES behavior to the aadA reporter gene product. (A) Map of the R12 fragment in wild-type and FKR12 strains. The positions of known genes and relevant restriction sites are indicated (S, StuI; RI, EcoRI) (31). (B) Percentage of strains with FKR12/mcd1, FKR12/WT, and FKR12/ccs1 genotypes, as indicated, growing on TAP (T0), T1, T2, or T3 medium, as indicated. Absolute numbers of growing clones (resistant clones) are indicated in C. The 62 FKR12/WT strains originated from three crosses—32 from FKR12 × WT, 14 from FKR12 × mcd1-F16, and 16 from FKR12 × ccs1-ac206—that yielded the same ratio of about 50% resistant clones on T2.