TABLE 2.
Effect of HCV CE1 proteins expression on DC maturation pathways
| Treatment | Transgene | Mean fluorescence with surface markera:
|
|||
|---|---|---|---|---|---|
| Isotype control | CD80 | CD86 | IAd | ||
| None | CE1 | 7 | 108 | 93 | 462 |
| NS3 | 7 | 139 | 129 | 552 | |
| LPS | CE1 | 6 | 220 | 136 | 846 |
| NS3 | 7 | 173 | 126 | 815 | |
| TNF-α | CE1 | 6 | 105 | 92 | 738 |
| NS3 | 8 | 186 | 169 | 1100 | |
| 3T3-SAMEN | CE1 | 7 | 86 | 79 | 519 |
| NS3 | 6 | 120 | 117 | 669 | |
| 3T3-CD40L | CE1 | 6 | 120 | 109 | 572 |
| NS3 | 5 | 163 | 152 | 942 | |
a
Bone marrow-derived DC were grown for 7 days with GM-CSF and IL-4 and then infected with AdCE1 or AdNS3. One day after infection, DC were incubated with LPS (1 μg/ml), TNF-α (200 ng/ml), or 3T3-CD40L fibroblasts to induce maturation. As a control, cells were left untreated or cultured in the presence of 3T3-SAMEN fibroblasts. After two additional days, surface marker expression was analyzed by flow cytometry. The numbers indicate the mean fluorescence values for each marker and are representative of three different experiments.