FIG. 1.
HCMV gB interacts with PACS-1 in a phosphorylation-dependent manner. GST-tagged wild-type HCMV gB cytoplasmic domain (with or without [+ or −] CK2 phosphorylation), mutant gB cytoplasmic domains containing S900D or S900A mutations, or GST alone were incubated with TRX-tagged PACS-1FBR. Protein complexes were recovered with glutathione-agarose, and extracts were analyzed for associated TRX-PACS-1FBR by Western blotting with TRX-specific antisera. Interaction signals from separate experiments were quantified by using National Institutes of Health gel analysis software and are shown as mean interactions ± the SD.