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. 2003 Oct;77(20):11105–11113. doi: 10.1128/JVI.77.20.11105-11113.2003

FIG. 3.

FIG. 3.

Depletion of endogenous PACS-1 protein by siRNA causes a mislocalization of HCMV gB. U373 cells were transfected with or without PACS-1-specific siRNA. (A) PACS-1 protein levels were assessed by Western blotting 3 days posttransfection with PACS-1-specific antisera. (B) FLAG-tagged HCMV gB was expressed in control (a to c) or PACS-1 siRNA-treated (d to f) cells by using adenovirus vectors at an MOI of 2 for 24 h. Cells were fixed and developed with antisera specific for the FLAG tag (M1; a and d) and TGN46 (b and e). M1 staining is shown in green, and TGN46 staining is shown in red.