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Far- and near-UV CD spectra of RICK-CARD and proline mutants. In all panels, the spectra of wild-type RICK-CARD (dashed lines) are compared with the RICK-CARD mutants (solid lines). (A) Spectra for RICK-CARD unfolded in 4 M urea-containing buffer (short dashed lines) are shown for comparison. The following spectra are shown: (A) P47A, (B) P85A, (C) P87A, (D) P47A/P85A, (E) P47A/P87A, (F) P85A/P87A, and (G) P3A.
