Figure 6.

Sequential mixing stopped flow studies. (A) Fluorescence signal versus delay time in sequential mixing double jump experiments for wild-type (○) and P87A (□) RICK-CARDs. (B) Half-times of the burst phase transitions for the proline mutants and for wild-type RICK-CARD. The half-times shown were estimated from the first transition obtained from data such as those shown in A. (C) Amplitudes of the fast (○) and slow (▵) refolding phases versus unfolding delay time for wild-type RICK-CARD. Amplitudes of the fast (□) refolding phase versus unfolding delay time for P87A RICK-CARD. The lines through the data are of no theoretical value, and are presented to guide the eye.