Table 1.
Time constants and amplitudes from refolding kinetics of RICK-CARD proline mutants
| Aburst (% total) | A1 (% total) | τ1 (sec) | A2 (% total) | τ2 (sec) | A3 (% total) | τ3 (sec) | τUN (sec) | mU-TS (kcal mol−1 M−1) | |
| Wild type | 65 | 16 ± 0.2 | 0.032 ± 0.0005 | 2 ± 0.3 | 1.0 ± 0.13 | 17 ± 0.1 | 83 ± 1 | 0.031 ± 0.006 | 0.90 ± 0.03 |
| Category I | |||||||||
| P47A | 75 | 23 ± 0.2 | 0.029 ± 0.0007 | 2 ± 0.1 | 8.3 ± 1.2 | 0.017 ± 0.003 | 0.81 ± 0.06 | ||
| P87A | 65 | 35 ± 0.3 | 0.039 ± 0.002 | 0.037 ± 0.003 | 0.79 ± 0.2 | ||||
| P47A/P85A | 85 | 15 ± 0.1 | 0.037 ± 0.0008 | 0.018 ± 0.002 | 0.85 ± 0.02 | ||||
| P3A | 89 | 11 ± 0.2 | 0.035 ± 0.002 | 0.016 ± 0.008 | 0.84 ± 0.1 | ||||
| Category II | |||||||||
| P85A | 100 | ||||||||
| P47A/P87A | 100 | ||||||||
| P85A/P87A | 100 | ||||||||
The data in Figure 4 were fit to a sum of one, two, or three exponentials, as described in the text. The data for wild-type RICK-CARD were reported previously (Chen and Clark 2003).