Figure 4.

Effects of peptide ligands on wtHop secondary structure. Far UV-CD measurements were obtained for purified recombinant wtHop (1 μM) in the presence or the absence of synthetic peptide (100 μM). The peptides used were a control SRMAAVD (A), the Hsp90-related peptide SRMEEVD (B), or the Hsp70-related peptide PTIEEVD (C). Spectra were obtained for wtHop alone (solid circles), peptide alone (open circles), and the wtHop+ peptide mixture (solid squares). Also, theoretical spectra were calculated by adding the spectra for wtHop alone and peptide alone (open squares). Measurements were made in triplicate at either pH 7.4 (left-hand panels) or pH 5 (right-hand panels); representative data from one of three replicate experiments are shown.