Table 1.
Kinetic and thermodynamic properties of FynSH3 and seven point mutants
| ln (kt)a | mf (kJ/mol.M) | ln (ku)a | mu (kJ/mol.M) | ΔGu (kJ/mol) | meq (kJ/mol.M) | |
| WT | 4.88 (0.08) | −4.73 (0.28) | −4.48 (0.19) | 1.78 (0.11) | 23.20 (0.35) | −6.51 (0.30) |
| V55T | 4.58 (0.38) | −4.72 (0.38) | −0.73 (0.16) | 1.79 (0.08) | 13.15 (1.06) | −6.51 (0.34) |
| V55M | 5.11 (0.28) | −4.93 (0.18) | −3.37 (0.35) | 1.86 (0.16) | 21.04 (1.48) | −6.78 (0.18) |
| V55A | 4.90 (0.42) | −4.90 (0.56) | −0.52 (0.27) | 1.62 (0.13) | 13.43 (0.46) | −6.52 (0.45) |
| V55G | 4.57 (0.29) | −4.12 (0.72) | 1.36 (1.02) | 1.81 (0.49) | 7.95 (3.25) | −5.93 (1.02) |
| I28V | 3.90 (0.18) | −4.62 (0.23) | −4.40 (0.37) | 1.71 (0.16) | 20.58 (0.85) | −6.34 (0.28) |
| I28L | 4.08 (0.29) | −4.75 (0.32) | −4.08 (0.94) | 1.75 (0.14) | 20.22 (1.85) | −6.50 (0.21) |
| I28A | 1.80 (0.12) | −4.71 (0.46) | −2.77 (0.14) | 1.54 (0.14) | 11.34 (0.64) | −6.25 (0.32) |
Mean and standard deviations observed across three laboratories.
a Extrapolated rates in the absence of denaturant.