Table 2.
Pairwise φ values
| Extrapolated | UT valuesa | ||
| Proteins | φ (σ) | ΔΔGu (σ) | φ |
| Wt-I28A | 0.64 (0.04) | 11.9 (0.7) | 0.71 |
| Wt-I28L | 0.87 (0.75) | 3.0 (1.6) | 0.25 |
| Wt-I28V | 0.98 (0.44) | 2.6 (0.6) | 1.50 |
| Wt-V55A | −0.01 (0.09) | 9.8 (0.8) | 0.01 |
| Wt-V55M | −0.84 (1.24) | 2.2 (1.6) | |
| Wt-V55T | 0.07 (0.06) | 10.1 (1.2) | |
| Wt-V55G | 0.05 (0.03) | 15.3 (3.3) | |
| I28A-I28L | 0.66 (0.21) | 8.9 (1.6) | 0.98 |
| I28A-I28V | 0.56 (0.06) | 9.2 (0.7) | 0.66 |
| I28A-V55A | 3.99 (1.47) | 2.1 (0.7) | 3.62 |
| I28A-V55M | 0.85 (0.04) | 9.7 (0.9) | |
| I28A-V55T | 3.93 (0.62) | 1.8 (0.5) | |
| I28A-V55G | −6.32 (8.55) | 3.4 (2.6) | |
| I28L-I28V | 0.34 (1.37) | 0.4 (1.0) | 0.04 |
| I28L-V55A | −0.34 (0.25) | 6.8 (2.2) | −0.21 |
| I28L-V55M | 0.94 (2.24) | 0.8 (1.8) | |
| I28L-V55T | −0.19 (0.22) | 7.1 (1.8) | |
| I28L-V55G | −0.12 (0.15) | 12.3 (3.0) | |
| I28V-V55A | −0.37 (0.24) | 7.2 (1.2) | −0.12 |
| I28V-V55M | 1.55 (3.09) | 0.5 (1.3) | |
| I28V-V55T | −0.24 (0.20) | 7.4 (1.0) | |
| I28V-V55G | −0.15 (0.13) | 12.6 (2.9) | |
| V55A-V55M | 0.07 (0.08) | 7.6 (1.4) | |
| V55A-V55T | 0.49 (1.01) | 0.3 (1.0) | |
| V55A-V55G | 0.19 (0.16) | 5.5 (3.1) | |
| V55M-V55T | 0.17 (0.04) | 7.9 (0.4) | |
| V55M-V55G | 0.10 (0.02) | 13.1 (1.8) | |
| V55T-V55G | 0.01 (0.08) | 5.2 (2.2) | |
Mean and standard deviations across three laboratories; ΔΔGU in kJ/mol.
aValues calculated from kinetic data reported in Northey et al. (2002).