Table 3.

Change in folding free energies and the standard deviations of triplicate φ

	Extrapolated		Nonzero		Fixed-m
Mutation	ΔΔGU	σ (φ)	Δ ΔGU	σ (φ)	Δ ΔGU	σ (φ)
Wt-I28L	2.98	0.74	2.86	0.47	2.86	0.49
Wt-I28V	2.62	0.44	2.17	0.13	2.14	0.14
Wt-V55A	9.78	0.09	9.13	0.05	9.15	0.06
Wt-V55M	2.17	1.27	2.73	0.12	2.86	0.01
Wt-V55T	10.07	0.06	10.08	0.03	10.11	0.02
Wt-V55G	15.26	0.03	14.77	0.04	15.05	0.02
I28A-I28L	8.88	0.21	7.79	0.25	7.61	0.25
I28A-I28V	9.24	0.06	8.48	0.09	8.34	0.09
I28A-V55A	2.08	1.45	1.52	0.91	1.32	1.82
I28A-V55M	9.70	0.04	7.92	0.06	7.62	0.05
I28A-V55T	1.79	0.61	0.57	10.63	0.37	60.88
I28A-V55G	3.40	8.73	4.12	0.49	4.57	0.25
I28L-I28V	0.36	1.35	0.68	0.71	0.72	0.57
I28L-V55A	6.80	0.25	6.27	0.14	6.29	0.13
I28L-V55M	0.82	2.24	0.12	8.63	0.01	32.27
I28L-V55T	7.08	0.22	7.22	0.16	7.25	0.15
I28L-V55G	12.28	0.15	11.91	0.08	12.18	0.06
I28V-V55A	7.16	0.24	6.95	0.12	7.02	0.11
I28V-V55M	0.46	3.08	0.56	7.7	0.72	50.27
I28V-V55T	7.44	0.20	7.91	0.15	7.97	0.15
I28V-V55G	12.64	0.13	12.59	0.08	12.91	0.06
V55A-V55M	7.61	0.08	6.4	0.08	6.30	0.08
V55A-V55T	0.29	1.00	0.95	0.4	0.96	0.33
V55A-V55G	5.48	0.17	5.64	0.03	5.89	0.04
V55M-V55T	7.90	0.04	7.35	0.01	7.25	0.03
V55M-V55G	13.10	0.02	12.04	0.05	12.19	0.03
V55T-V55G	5.20	0.08	4.69	0.16	4.93	0.08

Mean ΔΔG_U as determined using the listed methods (from rates extrapolated to zero denaturant, etc.) in kJ/mol. σ represents the standard deviation of our sets of three independent φ value determinations.