FIG. 6.
Growth kinetics of plaque-purified rec-RSV isolates 1-1 and 1-2 compared to wt rA2, ΔNS1/2, and ΔG/HEK. Triplicate cultures of HEp-2 (a) or Vero (b) cells were infected at an MOI of 0.01 PFU/cell. Supernatants were harvested daily and analyzed by plaque assay on Vero cells. Plaques were visualized by immunostaining with RSV F-specific antibodies. Mean titers (log10 PFU per milliliter) are shown with twice the standard error on either side of the mean.