Figure 4.
Bcl-XL interacts with caspase-9 and Apaf-1. (A) Bcl-XL interacts with caspase-9. (Upper) Western blot analysis of immunoprecipitated Bcl-XL and coimmunoprecipitated pro-caspase-9 and processed form (p35). (Lower) The expression of caspase-9 in total lysate. (B and C) Bcl-XL interacts with Apaf-1. 293T cells were transfected with indicated plasmids and the lysates immunoprecipitated with anti-Flag (B) or anti-Myc (C) antibody. WT, full-length Apaf-1-Myc; N, N-terminal Apaf-1-Myc; C, C-terminal Apaf-1-Myc. Panels show Western blot analysis of coimmunoprecipitated Apaf-1 and Bcl-XL proteins. Asterisk indicates nonspecific band.