Skip to main content
. 2003 Oct;77(20):10769–10779. doi: 10.1128/JVI.77.20.10769-10779.2003

FIG. 6.

FIG. 6.

dsRNA response in parental Huh7 and HeLa cells and HCV replicon-containing GS4.1 and SL1 cells. (A) Phosphorylation of eIF-2α. Huh7, GS4.1, HeLa, and SL1 cells were left untreated (lanes 1, 5, 9, and 13) or treated with 100 IU of IFN-α/ml for 12 h (lanes 2, 4, 6, 8, 10, 12, 14, and 16) and then transfected with poly(I:C) and incubated for 3 h (lanes 3, 4, 7, 8, 11, 12, 15, and 16). eIF-2α-P and total eIF-2α were determined by Western blots analysis with a monoclonal antibody specific for eIF-2α-P and an antibody specific for total eIF-2α protein. (B) Induction of IFN-β mRNA by dsRNA and IFN-α. Parental Huh7 and HeLa cells and HCV replicon-containing GS4.1 and SL1 cells were left untreated (lanes 1, 5, 9, and 13) or treated with 100 IU of IFN-α/ml (lanes 3, 4, 7, 8, 11, 12, 15, and 16) for 12 h and then transfected with poly(I:C) (lanes 2, 4, 6, 8, 10, 12, 14, and 16) for 3 h. An RNase protection assay was performed with probes specific for IFN-β and β-actin mRNAs.