TABLE 1.

Analysis of patterns of nonsynonymous variation in DENV at different levels of evolutionary divergence

Data seta	DENV type (place and date of collection)	No. of sequences	Length (bp)	π	pN	dN/dSb
Intrahost
1	3	180	318	0.003	0.757	1.062
2	3	180	366	0.004	0.732	1.183
3	3	69	393	0.003	0.747	1.262
4	3	20	1,479	0.002	0.520	0.562
Average				0.003	0.689	1.017
Interhost (epideniologically     linked populations)
5	2 (Thailand, 1987)	19	1,485	0.008	0.067	0.030
6	2 (Venezuela, 1997-2000)	35	1,485	0.010	0.133	0.066
7	2 (Vietnam, 1997-1998)	16	1,485	0.017	0.164	0.077
8	3 (Venezuela, 2000-2001)	15	1,479	0.002	0.200	0.131
Average				0.009	0.141	0.076
Interhost (among genotypes)
9	2	25	1,485	0.090	0.120	0.054
10	3	21	1,479	0.081	0.148	0.076
Average				0.086	0.134	0.065

^aSequences in data sets 1 and 2 were from capsid and NS2B genes, respectively. Sequences in all other data sets were from E genes. Data for each of sets 1 and 2 were collected from 18 patients; data for set 3 were collected from 6 patients.

^bFor data sets 1 to 3, d_N/d_S was calculated by dividing the numbers of nonsynonymous and synonymous mutations in samples from each patient by the average number of nonsynonymous and synonymous sites calculated across all patients. In individual pairwise comparisons in which there were no synonymous substitutions, d_N/d_S was set at 1.0.