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. 2001 Jan;10(1):187–199. doi: 10.1110/ps.29201

Fig. 5.

Fig. 5.

Fig. 5.

Fig. 5.

Fig. 5.

(A) Reversed phase chromatography of V8 protease digestion of PER apoA1. The peptide indicated as 170–179* has Ser in position 174 and peptide 170–179 has Leu in position 174. (B) Detail of V8 protease digest of apoA1 of patient PIC. Peptides 170–179* and 170–179 are indicated. (C) Mass spectrum of peptide 170–179* (eluted at 10.77 min) and peptide 170–179 (eluted at 15.82 min) from PER apoA1 shown in panel A. (D) Mass spectrum of peptide 170–179* (eluted at 10.50 min) and peptide 170–179 (eluted at 15.28 min) from PIC apoA1 shown in panel B. (Inset) SDS-PAGE analysis of plasma apoA1 stained by Coomassie blue. (1) apoA1 purified from plasma and used for V8 protease digestion; (2) molecular weight standards (phosphorylase b = 97.4 kD, bovine serum albumin = 66.2 kD, ovalbumin = 45.0 kD, carbonic anhydrase = 31.0 kD, soybean trypsin inhibitor = 21.5 kD, lysozyme = 14.4 kD).