FIG. 6.

Binding of HR2 peptides to HN sequences with single amino acid changes. Different concentrations of GST, GST-124B, GST-124B-I133L, and GST-124B-L140A fusion proteins were blotted on Immobilon P as described in the legend to Fig. 2. Peptide HR2b-B was added to the blot, and binding was detected as described in the legend to Fig. 2 and Materials and Methods. The amount of binding was quantitated as described in the legends to Fig. 3 and 4. Results are the averages of three separate experiments.