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Resonance Raman spectra at room temperature of SBP in 0.1 M bicine buffer pH 7.0, 1 mM CaCl2 (a), and of a single crystal (b). Experimental conditions: 5 cm-1 spectral resolution (a) 514.5 nm excitation: 70 mW laser power at the sample, 45 s/0.5 cm-1 accumulation time; (b) 50 μW laser power on the crystal surface, 1560 s accumulation time.
