Fig. 2.

TM Finder prediction for M13 major coat protein, showing hydrophobicity (red trace) and helicity (blue trace). The sequence of the protein is ¹AEGDDPAKAA ¹¹FNSLQASATE ²¹YIGYAWAMVV ³¹VIVGATIGIK ⁴¹LFKKFTSKAS⁵⁰. M13 major coat protein contains a single transmembrane segment that may extend maximally from 25–45 as deduced from nuclear magnetic resonance studies (Papavoine et al. 1998). Application of window size = 19 AA predicts the TM segment to occur at residues 19–38, while using a smaller window size = 7, the TM segment is identified at 23–42, closer to its experimentally determined location.