Fig. 3.

CD thermal denaturation profile analysis. Fit of the CD thermal denaturation profile of TK_HSV1 (0.4 mg/mL) in TBSE containing 1 mM dT, as representative of the whole set of measured denaturation curves, to the two-state unfolding model described by Eq. 4b. (A) The open circles represent the CD signal of TK_HSV1 at 223 nm as a function of temperature. The solid line is the result of a nonlinear fit routine, fitting the unfolding CD data using Eq. 4b. The inset shows the fit results for the slope m_N and intercept Y_N of the native protein baseline, the melting temperature T_m, the enthalpy change ΔH between the unfolded and the native state (considered only as a mathematical fit parameter, not as thermodynamic value), and slope m_U and intercept Y_U of the unfolded protein baseline. (B) Residuals of the data fit shown in A representing the difference between the theoretical function and the actual data points.