FIG. 5.
The C-terminal domain of the G1 protein cytoplasmic tail directs polyubiquitination. COS7 cells were transfected with Cyto (lanes 1 and 2), Cyto-FY (lanes 3 and 4), Cyto-YF (lanes 5 and 6), and ΔC51 (lanes 7 and 8) constructs simultaneously with His6-ubiquitin in the presence (+) or absence (−) of the proteasome inhibitor ALLN. Cells were lysed, and equal amounts of total protein from straight lysates (A) or samples from Ni-NTA agarose precipitations (B) were run on SDS-PAGE and were Western blotted (WB) with Gal4 antibody.