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. 2003 Oct;77(20):10808–10818. doi: 10.1128/JVI.77.20.10808-10818.2003

FIG. 2.

FIG. 2.

Nuclease-hypersensitive sites of c-strand and v-strand for AbMV DNA A. Nuclei (+Protein) and protein-free DNA (−Protein) as a control were incubated with DNase I for different times. +Protein: lane 1, without DNase I, 60 sec; lanes 2 to 4, with DNase I, 20, 40, or 60 sec. −Protein: lane 1, without DNase I, 45 sec; lanes 6 to 8, with DNase I, 15, 30, or 45 sec. (c) The c-strand was analyzed with reference to nucleotide 1336 (LMPCR with primer P3a, b). (v) The v-strand with reference to nucleotide 1297 (LMPCR with primer P4a, b). Left side: Ethidium bromide (EtBr)-stained 1.8% agarose gel. Right side: Blotted DNA after hybridization with primer 3c or 4c. Lane M, size markers (Gibco BRL). Column B indicates the calculated fragment sizes corresponding to the bands marked with arrowheads. 1c and -v and 2c and -v and shaded arrows, hypersensitive sites for comparison with Fig. 4.