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. 1998 Apr 14;95(8):4398–4403. doi: 10.1073/pnas.95.8.4398

Figure 1.

Figure 1

RNase protection analyses of Siamois expression in animal cap explants. (A) Increasing doses of β-catenin mRNA (in picograms) were injected into two-cell stage eggs. Animal caps were explanted at the mid-blastula stage and cultured to the early gastrula (stage 10.25). Siamois is expressed in response to doses of 150 pg and above of β-catenin mRNA, and the FGF-R is present as a loading control. (B) BVg-1 mRNA (100 pg) was injected into animal caps; explants were taken at the mid-blastula stage and cultured until the very early gastrula (stage 10). The mesodermal marker genes Xgsc, Chd, Eomes, and Xbra are shown as positive controls for Vg-1 activity. (C) Increasing doses of SMAD2 mRNA (0.5–4.0 ng) or 2 ng of SMAD1 mRNA were injected into animal caps, and explants were taken at the mid-blastula stage; these were cultured to the early gastrula (stage 10.25). Expression in one whole embryo equivalent (WE) is shown as a positive control and uninjected animal caps (UN) as negative controls. In all cases, 10 animal cap explants were analyzed per injection.