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. 1998 Apr 14;95(8):4398–4403. doi: 10.1073/pnas.95.8.4398

Figure 5.

Figure 5

The injection of GSK-3β mRNA in the dorsal sub-equatorial region of embryos represses the expression of the SMAD2 responsive organizer genes Xgsc and Chd. (A) RNase protection analysis of whole embryos injected sub-equatorially with 500 pg of GSK-3β mRNA in either the ventral (V) or dorsal (D) blastomeres at the four-cell stage and then cultured to the early gastrula stage (stage 10.25). The reduced level of Sia and Xnr-3 expression on the dorsal side shows that GSK-3β is active. (B) Quantitation of these gel analyses shows the levels of expression, relative to FGF-R loading control, of the genes Xgsc, Chd, and Eomes; error bars are shown for six independent analyses.