FIG. 6.

Memory proliferative response after immunization using CpG DNAs as the adjuvant. Mice were injected in both lower flanks with OVA with or without DNA or ODN-PT as the adjuvant in PBS or IFA. On days 10 to 12, three to four replicate splenocyte cultures containing various concentrations of OVA were used to measure proliferative memory responses. (A) Memory after immunization with OVA alone (triangles), OVA with nCpG-PT (circles), and OVA with CpG-PT (squares) either as solutes in PBS (gray lines) or emulsified in IFA (black lines). (B) Mice were immunized with OVA in IFA with either HSV-1 DNA (squares) or CV-1 DNA (circles). (C) Mice were immunized with OVA in IFA with ICP27-PT (squares), gD-PT (circles), CpG-PT (diamonds), or nCpG-PT (triangles). Stimulation indices are normalized to medium controls for each set of mice (two to three per group). Each panel is representative of two experiments.