TABLE 3.
CpG ODN as adjuvant affects antibody isotope selection
| ODN | Immunizationa
|
OVA-specific antibody titerb
|
||||
|---|---|---|---|---|---|---|
| IFA | CFA | OVA | IgG2a | IgG1 | IgG2a/IgG1 ratio | |
| nCpG | + | − | + | 1:128 | 1:512 | 1:4 |
| CpG | + | − | + | 1:2,048 | 1:1,024 | 2:1 |
| gD | + | − | + | 1:2,048 | 1:1,024 | 2:1 |
| ICP27 | + | − | + | 1:4,096 | 1:1,024 | 4:1 |
| CFA control | NAc | + | + | 1:16,384 | 1:2,048 | 8:1 |
a
Mice were immunized in the rear footpad with 100 mg of OVA and either ODN, IFA, or CFA as the adjuvant.
b
On day 12 postimmunization, serum was collected and OVA-specific antibody titers were determined by ELISA. Titers are reported as the highest dilution with an absorbance value greater than the background level.
c
NA, not applicable.