FIG. 3.

Enhanced IL-2 production mediated by p12^I is calcium pathway dependent. Transduced Jurkat T cells were treated with BAPTA-AM (BAPTA) or cyclosporine before the T-cell receptor ligation mimicked by anti-CD3 (A) or with anti-CD28 stimulation (B). p12^I-transduced Jurkat T cells secreted significantly higher levels of IL-2 than did p12(AS)-transduced cells or mock-infected cells in the presence of anti-CD3 alone (A) or with anti-CD28 stimulation (B). BAPTA-AM and cyclosporine treatment resulted in significantly reduced IL-2 production in p12^I- and p12(AS)-transduced cells, as well as in mock-infected cells. However, IL-2 production in p12^I-expressing cells after the addition of either inhibitor was not significantly different from that seen in p12(AS)-transduced cells or in mock-infected cells. Values are the means of triplicate or six samples. Statistical significance was analyzed by using the Student t test. ✽, P < 0.05.