FIG. 4.

p12^I promotes NFAT activation in response to CD3 with or without CD28 stimulation. Transduced Jurkat T cells transfected with pNFAT-Luc (A) or AP-1-Luc (B) plasmid were stimulated with either PMA, PMA plus ionomycin (P+I), anti-CD3, or anti-CD3 plus anti-CD28. The graph depicts the fold induction of NFAT or AP-1 luciferase activity in cells expressing p12^I (p12) versus those transduced with antisense p12 (AS). Datum points are the means of triplicate samples from three independent experiments.