FIG. 8.

Hyperactivated p12^I truncation mutants induce NFAT activation in the same fashion as wild-type p12^I. Jurkat T cells were transfected with plasmids containing different mutants in combination with pNFAT-Luc plasmid, and cells were treated with cyclosporine (A), BAPTA-AM (B), or U0126 (C) for 30 min before PMA was added. These inhibitors significantly reduced NFAT activation mediated by the wild type and the hyperactivated mutants 15-99 and 15-69. Datum points are the means of three independent transfection experiments. Statistical significance was analyzed by using the Student t test.