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Southern blots of restriction enzyme digestion products of the DNA of the E. hirae recombinants. The E. hirae ATCC 9790 (9790) and R40 strains, as well as pDML540 (6.9-kb EcoRI insert from R40) and the Smart ladder (MWM), were used as controls. (A) EcoRI digestion products probed with O15 (pbp5). (B) PstI digestion products probed with O16* (psr). (C) PvuII digestion products probed with O15 (pbp5). (D) BglII (lanes B) and NcoI (lanes N) digestion products hybridized with the Kmr probe.
