TABLE 1.
MICs of FQs for various N-terminal ParE mutants of S. pneumoniae
| Straina | ParE amino acid mutation at the following positionb:
|
MIC (μg/ml) of the following FQc:
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 15 | 24 | 28 | 31 | 43 | 103 | SPX | GRE | MFX | CIP | GAT | NOV | |
| R6 | A | A | R | M | H | H | 0.25 | 0.5 | 0.125 | 1 | 0.5 | 0.5 |
| TR6 | 1 | 2 | 0.5 | 1.5 | 1 | 0.5 | ||||||
| TR6 17d | H | 4 | 8 | 1.5 | 1.5 | 1 | 0.25 | |||||
| TR6 II1d | C | 3 | 8 | 1.5 | 1.5 | 1 | 0.25 | |||||
| TR6 I1d | L | 4 | 8 | 1.5 | 1.5 | 1 | 0.25 | |||||
| TR6 I2d | V | V | 3 | 6 | 1.5 | 1.5 | 1 | 0.25 | ||||
| TR6 I4d | V | L | 3 | 6 | 1.5 | 1.5 | 1 | 0.25 | ||||
| TR6 II2d | V | R | 3 | 6 | 1.5 | 1.5 | 1 | 0.25 | ||||
| TR6 II4d | Y | 3 | 8 | 1.5 | 1.5 | 1 | 0.25 | |||||
| TR6 M1e | Y | 3 | 8 | 1.5 | 1.5 | 1 | 0.25 | |||||
| TR6 M2e | F | 3 | 6 | 1.5 | 1.5 | 1 | 0.25 | |||||
a
R6 is a wild-type S. pneumoniae strain; all of the mutants harbor the GyrA (Ser81Phe) mutation present in TR6; only one mutant representative of each class is presented.
b
ParE positions are those from reference 37. Only amino acids different from those in wild-type pneumococcal strain R6 are shown.
c
SPX, sparfloxacin; GRE, grepafloxacin; MFX, moxifloxacin; CIP, ciprofloxacin; GAT, gatifloxacin; NOV, novobiocin.
d
Mutant obtained by PCR-based hypermutagenesis.
e
Mutant obtained by site-directed mutagenesis.